Duplication of genes encoding non‐clathrin coat protein γ‐COP in vertebrate, insect and plant evolution

FEBS Letters

Rhee, Myungchull²  Chung, Jae Hoon³  Lee, Young Jae³  Hahn, Yoonsoo³  Yang, Seung Kyoung³  Mita, Kazuei⁴  Yun, Ji Hye³  Huh, Tae-Lin¹  Park, Chang Won³ 

[1] Department of Genetic Engineering, Kyungpook National University, Taegu 702-701, South Korea;Department of Biology, Chungnam National University, Taejon 305-764, South Korea;Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea;Genome Research Group, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan

关键词: Non-clathrin coat protein γ-COP;    Copg;    Gene duplication;    Phylogenetic analysis;   

DOI  :  10.1016/S0014-5793(00)02033-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Coatomer is a major component of COPI vesicles and consists of seven subunits. The γ-COP subunit of the coatomer is believed to mediate the binding to the cytoplasmic dilysine motifs of membrane proteins. We characterized cDNAs for Copg genes encoding γ-COP from mouse, zebrafish, Drosophila melanogaster and Bombyx mori. Two copies of Copg genes are present in vertebrates and in B. mori. Phylogenetic analysis revealed that two paralogous genes had been derived from a single ancestral gene by duplication independently in vertebrates and in B. mori. Mouse Copg1 showed ubiquitous expression with the highest level in testis. Zebrafish copg2 was biallelically expressed in hybrid larvae in contrast to its mammalian ortholog expressed in a parent-of-origin-specific manner. A phylogenetic analysis with partial plant cDNA sequences suggested that copg gene was also duplicated in the grass family (Poaceae).

【 授权许可】

Unknown