DNA stability in plant tissues: implications for the possible transfer of genes from genetically modified food

FEBS Letters

Forbes, J.Michael¹  Chiter, Amar²  Blair, G.Eric² 

[1] School of Biology, University of Leeds, Leeds LS2 9JT, UK;School of Biochemistry and Molecular Biology, Room 8.10a, Garstang Building, University of Leeds, Mount Preston Street, Leeds LS2 9JT, UK

关键词: Rubisco;    Genetically modified organism;    DNA;    β-Lactamase gene;    Maize ribulose 1;    5-bisphosphate carboxylase/oxygenase small subunit;   

DOI  :  10.1016/S0014-5793(00)01986-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The potential for transfer of antibiotic resistance genes from genetically modified (GM) plant material to microbes through genetic recombination in the human or animal gut is a consideration that has engendered caution in the use of GM foods. This study was aimed at defining the optimal physical and chemical conditions necessary to ensure sufficient fragmentation of DNA in plant tissues to a size where it would be unlikely to be stably transferred to bacterial gut microflora. The ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit (Rubisco SS) genes are of similar size (approximately 1.4 kb) to transgenes present in GM plants. DNA analysis and PCR amplification of Rubisco SS genes showed that fresh maize and maize silage contained high molecular weight DNA and intact Rubisco SS genes. Relatively high temperatures and pressurised steam were necessary to degrade fully genomic DNA and Rubisco SS genes in maize and wheat grains, the source of most animal feedstuffs. Furthermore, chemical expulsion and extrusion of oilseeds resulted in residues with completely degraded genomic DNA. These results imply that stringent conditions are needed in the processing of GM plant tissues for feedstuffs to eliminate the possibility of transmission of transgenes.

【 授权许可】

Unknown