Carboxyl‐terminal propeptide of type I collagen (c‐propeptide) modulates the action of TGF‐β on MC3T3‐E1 osteoblastic cells

FEBS Letters

Mizuno, Morimichi¹  Kuboki, Yoshinori¹  Fujisawa, Ryuichi¹ 

[1] Department of Biochemistry, School of Dentistry, Hokkaido University, Kita 13 Nishi 7, Sapporo 060-8586, Japan

关键词: c-Propeptide;    Transforming growth factor-β;    MC3T3-E1 osteoblastic cell;   

DOI  :  10.1016/S0014-5793(00)01900-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously we found that the carboxyl-terminal propeptide of type I collagen (c-propeptide) is a major secretory protein of MC3T3-E1 osteoblastic cells. In this study, we found that c-propeptide suppresses collagen synthesis and alkaline phosphatase activity of MC3T3-E1 osteoblastic cells at the early-differentiated stage in a dose dependent manner. Mature osteoblasts did not respond to c-propeptide. These findings imply that c-propeptide modulates the function of osteoblasts at an early differentiation stage. Transforming growth factor-β (TGF-β) is stored in bone and released from bone matrix after the resorption by osteoclasts. We investigated the effect of c-propeptide on the action of TGF-β, and found that it enhanced the effect of TGF-β. We conclude that c-propeptide is a physiological modulator of TGF-β in bone metabolism.

【 授权许可】

Unknown