Lactate transport in rat adipocytes: identification of monocarboxylate transporter 1 (MCT1) and its modulation during streptozotocin‐induced diabetes

FEBS Letters

Heyes, Richard R.¹  Watt, Peter W.¹  Hundal, Harinder S.¹  Hajduch, Eric¹ 

[1] Department of Anatomy and Physiology, MSI/WTB Complex, Dow Street, University of Dundee, Dundee DD1 5EH, UK

关键词: Mono carboxylate transporter 1 (MCT1);    MCT4;    Insulin;    Membrane;    Diabetes;    Gluconeogenesis;   

DOI  :  10.1016/S0014-5793(00)01889-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have characterised L-lactate transport in rat adipocytes and determined whether these cells express a carrier belonging to the monocarboxylate transporter family. L-Lactate was taken up by adipocytes in a time-dependent, non-saturable manner and was inhibited (by ∼90%) by α-cyano-4-hydroxycinnamate. Lactate transport was stimulated by 3.7-fold upon lowering extracellular pH from 7.5 to 6.5 suggesting the presence of a lactate/proton-cotransporter. Antibodies against ono arboxylate ransporter 1 (MCT1) reacted positively with plasma membranes (PM), but not with intracellular membranes, prepared from adipocytes. MCT1 expression was down-regulated in PM of adipocytes from diabetic rats, which also displayed a corresponding loss (∼64%) in their capacity to transport lactate. The data support a role for MCT1 in lactate transport and suggest that changes in MCT1 expression are likely to have important implications for adipocyte lactate metabolism.

【 授权许可】

Unknown