Annexin XI may be involved in Ca2+‐ or GTP‐γS‐induced insulin secretion in the pancreatic β‐cell

FEBS Letters

Niwa, Tae¹  Iino, Satoshi²  Niki, Ichiro¹  Hidaka, Hiroyoshi¹  Sudo, Toshiki¹  Fukasawa, Tatsuya¹ 

[1] Department of Pharmacology, Nagoya University School of Medicine, 65 Tsuruma-cho, Nagoya 466-8550, Japan;First Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan

关键词: Annexin;    Insulin release;    Ca2+;    GTP-γS;    Pancreatic β-cell;   

DOI  :  10.1016/S0014-5793(00)01877-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aim of this study was to investigate possible involvement of annexin XI in the insulin secretory machinery. In fluorescence immunocytochemistry, annexin XI was found in the cytoplasm of pancreatic endocrine cells and a pancreatic β-cell line, MIN6, in a granular pattern. MIN6 cells also possessed weak and diffused annexin XI immunoreactivity in the cytoplasm. Immunoelectron microscopy revealed annexin XI in the insulin granules. Insulin secretion from streptolysin-O-permeabilized MIN6 cells was inhibited by anti-annexin XI antibody, when the release was stimulated by either Ca²⁺ or GTP-γS, but not by a protein kinase C-activating phorbol ester. Inhibition of insulin release by anti-annexin XI antibody was reproduced in permeabilized rat islets. These findings suggest that annexin XI may be involved in the regulation of insulin secretion from the pancreatic β-cells.

【 授权许可】

Unknown