A rise in nuclear calcium translocates annexins IV and V to the nuclear envelope

FEBS Letters

Kuijpers, Gemma¹  Pollard, Harvey B.¹  Rojas, Eduardo¹  Raynal, Patrick¹ 

[1] Laboratory of Cell Biology and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Annexin;    Ca2+;    Nucleus;    85-kDa cytosolic phospholipase A2;   

DOI  :  10.1016/0014-5793(96)00827-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Following incubation of human fibroblasts with Ca²⁺ ionophore A23187, we found strong immunofluorescence labelling of the nuclear envelope by annexin IV antibody. Using confocal imaging of cells loaded with Fluo-3, we showed that A23187 generates an intense and sustained rise of Ca²⁺ in the nucleus. By contrast, stimulation without extracellular Ca²⁺ produces only a brief rise in nuclear Ca²⁺ that does not promote annexin IV translocation to the nuclear envelope, and compounds that induce only a transient increase of nuclear Ca²⁺ do not support translocation of annexin IV. In addition, annexin V was also translocated to the nuclear envelope by A23187, but distribution of annexins I, II, VI and VII is unaffected. In in vitro assays with isolated nuclei, annexin V was also found to bind to the nuclear envelope in a Ca²⁺-dependent manner. These results demonstrate that the translocation to the nuclear envelope of different types of Ca²⁺-regulated proteins is directly triggered by a major rise of Ca²⁺ in the nucleus.

【 授权许可】

Unknown