Incorporation of N‐propanoylneuraminic acid leads to calcium oscillations in oligodendrocytes upon the application of GABA

FEBS Letters

Schmidt, Carolin²  Kettenmann, Helmut²  Reutter, Werner¹  Ohlemeyer, Carsten²  Horstkorte, Rüdiger¹ 

[1] Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, 14195 Berlin-Dahlem, Germany;Max Delbrück Centrum für Molekulare Medizin, Robert Rössle Str. 10, 13122 Berlin-Buch, Germany

关键词: Biochemical engineering;    Glycosylation;    N-Propanoylneuraminic acid;    N-Propanoylmannosamine;    Oligodendrocyte progenitor cell;    O4;   

DOI  :  10.1016/S0014-5793(00)01868-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sialylation of glycoproteins and glycolipids plays an important role during development, regeneration and pathogenesis. It has been shown that unnatural sialylation within glial cell cultures can have distinct effects on their proliferation and antigenic profiles. These cultures metabolize N-propanoylmannosamine (N-propanoylneuraminic acid precursor=P-NAP), a synthetic non-physiological precursor of neuraminic acid, resulting in the expression of N-propanoylneuraminic acid in glycoconjugates of their cell membranes [Schmidt, C., Stehling, P., Schnitzer, J., Reutter, W. and Horstkorte, R. (1998) J. Biol. Chem. 273, 19146–19152]. To determine whether these biochemically engineered sialic acids influence calcium concentrations in cells of the oligodendrocyte lineage, mixed glial cultures of oligodendrocytes growing on top of an astrocyte monolayer were exposed to glutamate, histamine, adrenaline, γ-aminobutyric acid (GABA), high potassium (high K⁺) and ATP. Calcium responses in P-NAP-treated oligodendrocytes were determined by confocal microscopy with the calcium indicator fluo-3 AM, and compared with control cultures. We showed that P-NAP differentially modulated the calcium responses of individual oligodendrocytes when GABA was applied. GABA induced calcium oscillations with up to four spikes per min in 60% of oligodendrocytes when treated with P-NAP.

【 授权许可】

Unknown