Cholestan‐3β,5α,6β‐triol, but not 7‐ketocholesterol, suppresses taurocholate‐induced mucin secretion by cultured dog gallbladder epithelial cells

FEBS Letters

Klinkspoor, J.Henriëtte¹  Wrenn, Steven P.²  Kuver, Rahul¹  Kaler, Eric W.²  Lee, Sum P.¹  Yoshida, Tadashi¹ 

[1] Department of Medicine, University of Washington School of Medicine and the Department of Veterans Affairs Medical Center, Seattle, WA, USA;Department of Chemical Engineering, Center for Molecular and Engineering Thermodynamics, University of Delaware, Newark, DE, USA

关键词: Epithelial cell;    Gallbladder;    Cytotoxicity;    Mucin secretion;    Oxysterol;    Cholesterol;    7KC;    7-ketocholesterol;    TriolC;    cholestan-3β;    5α;    6β-triol;    DGBE;    dog gallbladder epithelial;    HPAE;    human pulmonary artery endothelial;   

DOI  :  10.1016/S0014-5793(00)01831-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3β,5α,6β-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects in cultured human pulmonary artery endothelial cells (HPAE). Oxysterols inhibited cell proliferation in a dose-dependent fashion. Oxysterols inhibited cell growth to 50% of control at a higher dose for DGBE cells than for HPAE cells. TriolC was more cytotoxic than 7KC. We also investigated the effect of oxysterols on bile salt-induced mucin secretion by DGBE cells. TriolC suppressed mucin secretion by DGBE cells, whereas 7KC did not. These findings support the hypothesis that biliary oxysterols affect gallbladder mucosal function.

【 授权许可】

Unknown