Inhibition of inducible nitric oxide synthesis by oxidized lipoprotein(a) in a murine macrophage cell line

FEBS Letters

Volf, Ivo¹  Koller, Elisabeth¹  Friedl, Roswitha¹  Moeslinger, Thomas¹  Spieckermann, Paul Gerhard¹  Brunner, Monika¹ 

[1] Institute for Physiology, Schwarzspanierstraße 17, 1090 Vienna, Austria

关键词: Nitric oxide;    Lipoprotein(a);    Mouse macrophage;    Atherosclerosis;    Inducible nitric oxide synthase;   

DOI  :  10.1016/S0014-5793(00)01825-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Increased plasma levels of human lipoprotein(a) (Lp(a)) are highly correlated with the development of atherosclerotic lesions. During our study, we investigated the effects of native and hypochlorite oxidized lipoprotein(a) (ox-Lp(a)) on nitric oxide production by the inducible nitric oxide synthase (iNOS) in lipopolysaccharide/interferon stimulated mouse macrophages (J774A.1). Ox-Lp(a) (0–2 μg/ml) induces a dose dependent inhibition of inducible nitric oxide synthesis. iNOS protein expression showed a dose dependent reduction as revealed by immunoblotting when cells were incubated with increasing amounts of ox-Lp(a). Ox-Lp(a) decreases iNOS mRNA synthesis as shown by reverse transcription-polymerase chain reaction. Ox-Lp(a) induced iNOS inhibition might contribute to the development of atherosclerotic lesions by reducing the anti-atherogenic effects of nitric oxide.

【 授权许可】

Unknown