Aquaporin 3 cloned from Xenopus laevis is regulated by the cystic fibrosis transmembrane conductance regulator

FEBS Letters

Pavenstädt, Hermann¹  Kunzelmann, Karl²  Greger, Rainer³  Schreiber, Rainer² 

[1] Department of Medicine, Division of Nephrology, Albert-Ludwigs-University, Freiburg, Germany;Department of Physiology and Pharmacology, University of Queensland, St. Lucia, Brisbane, Qld. 4072, Australia;Physiological Institute, Albert-Ludwigs-University Freiburg, Hermann-Herder-Straße 7, 79104 Freiburg, Germany

关键词: Aquaporin 3;    Cystic fibrosis transmembrane conductance regulator;    Cystic fibrosis;    Cloning;    Expression;    Osmotic water permeability;    Glycerol transport;    Electrolyte secretion;    Ion transport;    Xenopus laevis oocyte;   

DOI  :  10.1016/S0014-5793(00)01689-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cystic fibrosis transmembrane conductance regulator (CFTR) is essential for epithelial electrolyte transport and has been shown to be a regulator of epithelial Na⁺, K⁺, and Cl⁻ channels. CFTR also enhances osmotic water permeability when activated by cAMP. This was detected initially in Xenopus oocytes and is also present in human airway epithelial cells, however, the mechanisms remain obscure. Here, we show that CFTR activates aquaporin 3 expressed endogenously and exogenously in oocytes of Xenopus laevis. The interaction requires stimulation of wild type CFTR by cAMP and an intact first nucleotide binding domain as demonstrated for other CFTR–protein interactions.

【 授权许可】

Unknown