Secondary structure of the 5′‐region of PGY1/MDR1 mRNA

FEBS Letters

Beabealashvilly, Robert Sh.¹  Vlassov, Valentin V.²  Zenkova, Marina A.²  Kostenko, Elena V.² 

[1] Institute of Experimental Cardiology, Cardiology Scientific Centre, Russian Academy of Medical Sciences, Moscow 121552, Russia;Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russia

关键词: RNA structure;    PGY1/MDR1;    Antisense oligonucleotide;   

DOI  :  10.1016/S0014-5793(00)01650-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to identify the optimal target sites for antisense oligonucleotides in the human multiple drug resistance mRNA, the secondary structure of the 5′-terminal part of this mRNA (nucleotides 1–678) was investigated. By using results of probing with ribonucleases T1, ONE and V1 and results of computer simulations, a model of the 5′-region of the PGY1/MDR1 mRNA was built. The molecule is formed by three major domains comprising several hairpins separated by single-stranded fragments. The predicted single-stranded regions of the PGY1/MDR1 mRNA efficiently bind complementary oligonucleotides.

【 授权许可】

Unknown