Estimation of H2O2 gradients across biomembranes

FEBS Letters

Antunes, Fernando¹  Cadenas, Enrique¹ 

[1] Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles, CA 90089-9121, USA

关键词: Hydrogen peroxide;    Glutathione peroxidase;    Catalase;    Steady state;    Redox regulation;    Jurkat T-cell;    GPx;    glutathione peroxidase;    GSH;    glutathione;    GSSG;    glutathione disulfide;    P s;    permeability coefficient;   

DOI  :  10.1016/S0014-5793(00)01638-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When cells are exposed to an external source of H₂O₂, the rapid enzymatic consumption of H₂O₂ inside the cell provides the driving force for the formation of the gradient across the plasma and other subcellular membranes. By using the concepts of enzyme latency, the following gradients – formed after a few seconds following the exposure to H₂O₂ – were estimated in Jurkat T-cells: [H₂O₂]_cytosol/[H₂O₂]_peroxisomes=3; [H₂O₂]_{extracellular}/[H₂O₂]_cytosol=7. The procedure presented in this work can easily be applied to other cell lines and provides a quantitative framework to interpret the data obtained when cells are exposed to an external source of H₂O₂.

【 授权许可】

Unknown