Dissection of the maturation reactions of the [NiFe] hydrogenase 3 from Escherichia coli taking place after nickel incorporation

FEBS Letters

Magalon, Axel¹  Böck, August¹ 

[1] Lehrstuhl für Mikrobiologie der Universität München, Maria-Ward-Strasse 1a, 80638 Munich, Germany

关键词: [NiFe] hydrogenase;    Maturation;    Cellular location;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(00)01542-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The steps in the maturation of the precursor of the large subunit (pre-HycE) of hydrogenase 3 from Escherichia coli taking place after incorporation of both iron and nickel were investigated. Pre-HycE could be matured and processed in the absence of the small subunit but association with the cytoplasmic membrane required heterodimer formation between the two subunits. Pre-HycE formed a complex with the chaperone-like protein HypC in the absence of the small subunit and, in this complex, also incorporated nickel. For the C-terminal processing, HypC had to leave the complex since only a HypC-free, nickel-containing form of pre-HycE was a substrate for the maturation endopeptidase.

【 授权许可】

Unknown