A novel human processed gene, DAD‐R, maps to 12p12 and is expressed in several organs

FEBS Letters

Horelli-Kuitunen, Nina¹  Kuittinen, Tero²  Lindholm, Päivi²  Maris, John M.³  Eggert, Angelika³  Saarma, Mart²  Palotie, Aarno¹ 

[1] Department of Clinical Chemistry, University of Helsinki, Helsinki University Central Hospital, FIN-00290 Helsinki, Finland;Insitute of Biotechnology, Viikki Biocenter, P.O. Box 56 (Viikinkaari 9), FIN-00014 University of Helsinki, Finland;The Children's Hospital of Philadelphia, Division of Oncology, ARC 902A, 324 South 34th Street, Philadelphia, PA 19104-4318, USA

关键词: Processed gene;    Apoptosis;    N-Linked glycosylation;    Human neuroblastoma;    12p12;    Synpolydactyly;   

DOI  :  10.1016/S0014-5793(00)01358-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA of a processed gene of human DAD-1 (defender against apoptotic cell death) was cloned from the human neuroblastoma cell line SH-SY5Y. The genomic sequence of this novel processed gene, DAD-R, lacked introns and was flanked by 8 bp terminal repeats. RT-PCR showed that the transcript is expressed predominantly in testis, ovaries, pancreas, lung and skeletal muscle. DAD-R has several possible initiation codons, one of them producing an open reading frame comprising 75% of the DAD-1 gene. We determined the chromosomal localization of DAD-R as 12p11.2–12p12.1, an area linked to familial synpolydactyly and frequently amplified in a variety of cancers, including those of testis, ovaries, pancreas and lungs.

【 授权许可】

Unknown