Myocyte enhancer factor 2C upregulates MASH‐1 expression and induces neurogenesis in P19 cells

FEBS Letters

Wilton, Sharon¹  Skerjanc, Ilona S¹ 

[1] Department of Biochemistry, Medical Sciences Building, University of Western Ontario, London, Ont. N6A 5C1, Canada

关键词: Neurogenesis;    Embryonal carcinoma;    Transcription;    MEF2;    Mash-1;   

DOI  :  10.1016/S0014-5793(00)01438-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

MEF2C is a transcription factor expressed in neural lineages. After transient transfection, the MEF2 family of factors can act synergistically with the neural-specific transcription factor, MASH-1, and activate exogenous neural-specific promoters. To determine whether MEF2C is capable of modulating endogenous gene expression, P19 cell lines were analyzed that overexpressed MEF2C, termed P19[MEF2C] cells. Here we show that P19[MEF2C] cells differentiate into neurons when aggregated with ME₂SO. MEF2C-induced neurons expressed neurofilament protein, the nuclear antigen NeuN, as well as MASH-1. Our results indicate that MEF2C can directly or indirectly activate the expression of MASH-1, leading to neurogenesis.

【 授权许可】

Unknown