The cytotoxic action of Bax on yeast cells does not require mitochondrial ADP/ATP carrier but may be related to its import to the mitochondria

FEBS Letters

Šabová, L'udmila¹  Zeman, Igor²  Kiššová, Ingrid²  Polčic, Peter²  Kempná, Petra¹  Kolarov, Jordan² 

[1] Cancer Research Institute, Slovak Academy of Sciences, 833 91 Bratislava, Slovakia;Department of Biochemistry, Faculty of Sciences, Comenius University, 842 15 Bratislava, Slovakia

关键词: Apoptosis;    ADP/ATP carrier;    Bax;    Mitochondrion;    Yeast;    AAC;    ADP/ATP carrier;    GFP;    green fluorescent protein;    SM;    synthetic liquid medium;    ANT;    bovine adenine nucleotide translocator;    DAPI;    4′;    6-diamidino-2-phenyl-indole;    PTP;    permeability transition pore;    VDAC;    voltage-dependent anion channel;    MTP1;    mitochondrial telomere binding protein;   

DOI  :  10.1016/S0014-5793(00)01379-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of the expression of murine Bax protein on growth and vitality was examined in Saccharomyces cerevisiae and compared with the effect of Bax in mutant cells lacking functional mitochondria. The cytotoxic effect of Bax on yeast does not require functional oxidative phosphorylation, respiration, or mitochondrial proteins (ADP/ATP carriers) implicated in the formation of the permeability transition pore in mammalian mitochondria. In the wild type S. cerevisiae the expression of Bax does not result in a severe effect on mitochondrial membrane potential and respiration. On the basis of Bax induced differences in the fluorescence of green fluorescent protein fused to mitochondrial proteins, it is proposed that Bax may interfere with one essential cellular process in yeast: the mitochondrial protein import pathway that is specific for the proteins of the mitochondrial carrier family.

【 授权许可】

Unknown