Pressurization facilitates adenovirus‐mediated gene transfer into vein graft

FEBS Letters

Suzuki, Masatoshi¹  Ishizaka, Nobukazu¹  Minami, Kayo²  Nagai, Ryozo¹  Taguchi, Jun-ichi¹  Tsukamoto, Kazuhisa²  Ohno, Minoru¹ 

[1] Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan;Department of Metabolic Disease, University of Tokyo Graduate School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan

关键词: Vein graft;    Adenovirus;    Pressurization;    β-Galactosidase;    Hydrostatic pressure;   

DOI  :  10.1016/S0014-5793(00)01348-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated whether application of non-distending hydrostatic pressure facilitates gene transfer into vein grafts. An external jugular vein was placed in a chamber with 100 μl adenovirus solution at a titer of 10¹⁰ pfu/ml and was pressurized to up to 8 atm above ambient pressure for 10 min. Histochemical analysis demonstrated a positive transgene expression in all layers of the vessel wall. Gene transfer with 8 atm pressurization resulted in an approximately 50 times higher transgene expression than that without pressurization. Under 8 atm pressurization, the efficiency of gene transfer reached a plateau at 7.5 min. The application of hydrostatic pressure may improve the effectiveness of intraoperative genetic engineering of vein grafts.

【 授权许可】

Unknown