Characterization of the interactions between the glycine transporters GLYT1 and GLYT2 and the SNARE protein syntaxin 1A

FEBS Letters

López-Corcuera, Beatriz¹  Geerlings, Arjan¹  Aragón, Carmen¹ 

[1] Centro de Biologı́a Molecular ‘Severo Ochoa’, Facultad de Ciencias, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Cientı́ficas, 28049 Madrid, Spain

关键词: Glycine transport;    Neurotransmitter reuptake;    Syntaxin;    SNARE protein;    Protein regulation;    Membrane trafficking;    GLYT;    glycine transporter;    PAGE;    polyacrylamide gel electrophoresis;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(00)01297-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study we have examined the effect of the SNARE protein syntaxin 1A on the glycine transporters GLYT1 and GLYT2. Our results demonstrate a functional and physical interaction between both glycine transporters and syntaxin 1A. Co-transfection of syntaxin 1A with GLYT1 or GLYT2 in COS cells resulted in approximately 40% inhibition in glycine transport. This inhibition was reversed by the syntaxin 1A-binding protein, Munc18. Furthermore, immunoprecipitation studies showed a physical interaction between syntaxin 1A and both transporters in COS cells and in rat brain tissue. Finally, we conclude that this physical interaction resulted in a partial removal of the glycine transporters from the plasma membrane as demonstrated by biotinylation studies.

【 授权许可】

Unknown