Iron overload and gene expression in HepG2 cells: analysis by differential display

FEBS Letters

Cassani, Camilla¹  Ginelli, Enrico²  Barisani, Donatella¹  Conte, Dario¹  Meneveri, Raffaella² 

[1] Cattedra di Gastroenterologia, Padiglione Granelli 3 P, IRCCS Ospedale Maggiore, Via F. Sforza 35, 20122 Milan, Italy;Dipartimento di Biologia e Genetica per le Scienze Mediche, University of Milan, Via Viotti 5, 20133 Milan, Italy

关键词: Differential display;    Hepatocyte;    Antioxidant;    Apolipoprotein B;    Semaphorin cd100;    Aldose reductase;    IRE;    iron regulatory element;    4-HNE;    4-hydroxy-2;    3-nonenal;    Apo B100;    apolipoprotein B100 mRNA;    FAC;    ferric ammonium citrate;    TfR;    transferrin receptor;   

DOI  :  10.1016/S0014-5793(00)01280-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways.

【 授权许可】

Unknown