Use of an affinity proteomics approach for the identification of low‐abundant bacterial adhesins as applied on the Lewisb‐binding adhesin of Helicobacter pylori

FEBS Letters

Karlsson, Karl-Anders¹  Nilsson, Carol¹  Larsson, Thomas¹  Bergström, Jörgen¹ 

[1] Institute of Medical Biochemistry, Göteborg University, Box 440, SE-405 30 Göteborg, Sweden

关键词: Bacterial adhesin;    Low-abundance protein;    Proteomics;    Protein–carbohydrate interaction;    Helicobacter pylori;   

DOI  :  10.1016/S0014-5793(00)01270-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Microbial attachment to host cell surfaces is considered to be the first essential step for colonization and infection. In most known cases, attachment is mediated by a specific protein–carbohydrate interaction. We have used a carbohydrate-containing crosslinking probe to select bacterial surface adhesins for trypsin digestion, MALDI-TOF mass spectrometry and identification against genome sequence. The present paper describes this functional proteomics approach for identification of the recently cloned low-abundant Lewis^b-binding adhesin of Helicobacter pylori. Protein identification was obtained through the enrichment of approximately 300 fmol of adhesin from solubilized cells.

【 授权许可】

Unknown