A novel Drosophila serpin that inhibits serine proteases

FEBS Letters

Min, Gi-Sik¹  Hashimoto, Carl¹  Kemler, Doris¹  Han, Jin-Hua¹  Zhang, Hong-Yan¹ 

[1] Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA

关键词: Serpin;    Serine protease inhibitor;    Drosophila melanogaster;   

DOI  :  10.1016/S0014-5793(00)01224-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Serpins define a large protein family in which most members function as serine protease inhibitors. Here we report the results of a search for serpins in Drosophila melanogaster that are potentially required for oogenesis or embryogenesis. We cloned and sequenced ovarian cDNAs that encode six distinct proteins having extensive sequence similarity to mammalian serpins, including residues important in the serpin inhibition mechanism. One of these new serpins in recombinant form inactivates, and complexes with, trypsin-like proteases in vitro. To our knowledge, these results represent the first evidence for a serpin in Drosophila that functions as a serine protease inhibitor.

【 授权许可】

Unknown