Human mast cells take up and hydrolyze anandamide under the control of 5‐lipoxygenase and do not express cannabinoid receptors

FEBS Letters

Maccarrone, Mauro¹  Erba, Fulvio¹  Fiorucci, Laura¹  Finazzi-Agrò, Alessandro¹  Ascoli, Franca¹  Bari, Monica¹ 

[1] Department of Experimental Medicine and Biochemical Sciences, University of Rome, Tor Vergata, Via di Tor Vergata 135, 00133 Rome, Italy

关键词: Anandamide;    Endocannabinoid;    Inflammation;    Lipoxygenase;    Nitric oxide;    Tryptase;    AEA;    anandamide (arachidonoylethanolamide);    AM404;    N-(4-hydroxy-phenyl)-arachidonoylamide;    ATFMK;    arachidonoyl-trifluoromethyl-ketone;    CB1/2R;    type 1/2 cannabinoid receptor;    CP55.940;    5-(1;    1′-dimethyheptyl)-2-[1R;    5R-hydroxy-2R-(3-hydroxy-propyl)cyclohexyl]phenol;    ETYA;    5;    8;    11;    14-eicosatetraynoic acid;    FAAH;    fatty acid amide hydrolase;    GAR-AP;    goat anti-rabbit alkaline phosphatase conjugate;    HMC;    human mast cells;    MES;    2-[N-morpholino]ethanesulfonic acid;    MK886;    3-[1-(p-chlorobenzyl)-5-(isopropyl)-3-tert-butylthioindol-2-yl]-2;    2-dimethyl propanoic acid;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    PBS;    phosphate-buffered saline;    PEA;    palmitoylethanolamide;    RP-HPLC;    reversed phase high performance liquid chromatography;    SNAP;    S-nitroso-N-acetylpenicillamine;    SNP;    sodium nitroprusside;    TGPL;    N-p-tosyl-Gly-Pro-Lys-p-nitroanilide;   

DOI  :  10.1016/S0014-5793(00)01223-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human mast cells (HMC-1) take up anandamide (arachidonoyl-ethanolamide, AEA) with a saturable process (K _m=200±20 nM, V _max=25±3 pmol min⁻¹ mg protein⁻¹), enhanced two-fold over control by nitric oxide-donors. Internalized AEA was hydrolyzed by a fatty acid amide hydrolase (FAAH), whose activity became measurable only in the presence of 5-lipoxygenase, but not cyclooxygenase, inhibitors. FAAH (K _m=5.0±0.5 μM, V _max=160±15 pmol min⁻¹ mg protein⁻¹) was competitively inhibited by palmitoylethanolamide. HMC-1 cells did not display a functional cannabinoid receptor on their surface and neither AEA nor palmitoylethanolamide affected tryptase release from these cells.

【 授权许可】

Unknown