Anandamide activates human platelets through a pathway independent of the arachidonate cascade

FEBS Letters

Menichelli, Adriana²  Maccarrone, Mauro¹  Del Principe, Domenico²  Finazzi Agrò, Alessandro¹  Bari, Monica¹ 

[1] Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Via di Tor Vergata 135, I-00133 Rome, Italy;Department of Public Health and Cell Biology, University of Rome Tor Vergata, Via di Tor Vergata 135, I-00133 Rome, Italy

关键词: Anandamide;    Arachidonate;    Endocannabinoid;    Lipoxygenase;    Platelet;    AnNH;    anandamide (arachidonoylethanolamide);    CB1/2;    cannabinoid receptor 1/2;    PaNH;    palmitoylethanolamide;    FAAH;    fatty acid amide hydrolase;    PMSF;    phenylmethylsulfonyl fluoride;    ASA;    acetylsalicylic acid;    NO;    nitric oxide;    SNP;    sodium nitroprusside;    SNAP;    S-nitroso-N-acetylpenicillamine;    SPER/NO;    spermine NONOate;    (13-H)ODNHEtOH;    (13-hydroxy) linoleoylethanolamide;    15-/11-H(P)AnNH;    15-/11-hydro(pero)xy anandamide;    PBS;    phosphate-buffered saline;    RP-HPLC;    reversed phase high performance liquid chromatography;    GAR-AP;    goat anti-rabbit alkaline phosphatase conjugate;   

DOI  :  10.1016/S0014-5793(99)00308-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Anandamide (arachidonoylethanolamide, AnNH) is shown to activate human platelets, a process which was not inhibited by acetylsalicylic acid (aspirin). Unlike AnNH, hydroperoxides generated thereof by lipoxygenase activity, and the congener (13-hydroxy)linoleoylethanolamide, were unable to activate platelets, though they counteracted AnNH-mediated stimulation. On the other hand, palmitoylethanolamide neither activated human platelets nor blocked the AnNH effects. AnNH inactivation by human platelets was afforded by a high-affinity transporter, which was activated by nitric oxide-donors up to 225% of the control. The internalized AnNH could thus be hydrolyzed by a fatty acid amide hydrolase (FAAH), characterized here for the first time.

【 授权许可】

Unknown