Ephrin‐B2 is a candidate ligand for the Eph receptor, EphB6

FEBS Letters

Holien, Toril¹  Levy, Finn Olav²  Munthe, Else¹  Rasmussen, Anne-Marie¹  Aasheim, Hans-Christian¹  Rian, Edith¹ 

[1] Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway;MSD Cardiovascular Research Center and Institute for Surgical Research, University of Oslo, The National Hospital, Rikshospitalet, N-0027 Oslo, Norway

关键词: Tyrosine kinase;    Ligand;    Reh cell line;    Scatchard analysis;   

DOI  :  10.1016/S0014-5793(99)01793-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

No ligand has hitherto been designated for the Eph receptor tyrosine kinase family member, EphB6. Here, expression of an EphB6 ligand in the pro-B leukemic cell line, Reh, is demonstrated by binding of soluble EphB6-Fc fusion protein to the Reh cells. The ligand belongs to the subgroup of membrane spanning ligands, as suggested by the fact that phosphatidylinositol-specific phospholipase C treatment did not abrogate binding of EphB6-Fc. Two transmembrane Eph receptor ligands, ephrin-B1 and ephrin-B2, were identified in Reh cells. Analysis of EphB6-Fc fusion protein binding to ephrin-B1 or ephrin-B2 transfected COS cells revealed a high-affinity saturable binding between EphB6-Fc and ephrin-B2, but not with ephrin-B1. In mice, EphB6 has previously been shown to be expressed in thymus. Here, we show expression of EphB6 in human thymus, as well as the expression of ephrin-B2 in both human and mouse thymus. We conclude that ephrin-B2 may be a physiological ligand for the EphB6 receptor.

【 授权许可】

Unknown