Binding of cardiotoxin analogue III from Formosan cobra venom to FL cells

FEBS Letters

Takechi, Masayuki²  Hayashi, Kyozo¹  Tanaka, Yasuo² 

[1]Department of Biology, Gifu Pharmaceutical University, Gifu 502, Japan

[2]Faculty of Pharmacy, Kinki University, Higashiosaka 577 Japan

关键词: Cardiotoxin;    Cytotoxicity;    Acidic phospholipid;    (FL cell;    Cobra venom);    Scatchard analysis;    Binding equilibrium;    FL;    fetal lung;    CT;    cardiotoxin;   

DOI  :  10.1016/0014-5793(86)80882-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding equilibrium at 37 or 0°C of ¹²⁵I-cardiotoxin analogue III (CT III) to fetal lung (FL) cells (cultured human amnion cells) was achieved within 1 h, and the binding at 37°C was irreversible. The Scatchard analysis at 37°C on the binding of ¹²⁵I-CT III indicated that FL cells had two types of binding sites with different association constants. The association constant and the number of high-affinity sites was 1.1 × 10¹⁰ mol⁻¹ or 2.8 × 10⁶ per FL cell, respectively. At 37 or 0°C, the cytotoxicity of CT III paralleled the amount of bound CT III to FL cells, and at 37°C was inhibited by the presence of acidic phospholipids.

【 授权许可】

Unknown