Different transcriptional properties of mSim‐1 and mSim‐2

FEBS Letters

Moffett, Peter¹  Pelletier, Jerry¹ 

[1] Department of Biochemistry, McGill University, 3655 Drummond St., Montreal, Que. H3G 1Y6, Canada

关键词: Sim;    Arnt;    Transcription;    DNA binding;    Basic helix-loop-helix PAS;   

DOI  :  10.1016/S0014-5793(99)01750-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mSim-1 and mSim-2 gene products are mammalian homologues of the Drosophila Sim gene. The dSim gene product transactivates through a DNA binding site known as the CNS midline enhancer (CME) element. We have investigated the transcriptional properties of mSIM-1 and mSIM-2 mediated through the CME element in concert with their dimerization partners, ARNT and ARNT-2. The mSIM-1/ARNT heterodimer transactivates reporter constructs via the ARNT carboxy-terminus. However, mSIM-2 quenches ARNT transactivation. We find that mSIM-2 competes with mSIM-1 for binding to ARNT, suggesting a possible antagonism between these transcription factors.

【 授权许可】

Unknown