Distinct distal and proximal p53‐binding sites in the MCK promoter govern the transcriptional response to p53

FEBS Letters

Jackson, Paul¹  Yardley, Gina¹ 

[1] Oncology Research Centre, Prince of Wales Hospital, Randwick, NSW 2031, Australia

关键词: p53;    Muscle creatine kinase;    Promoter;    Transcription;    DNA binding;    Gene activation;   

DOI  :  10.1016/S0014-5793(97)00283-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter. P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each other. In contrast, co-operation between D-binding sites results in levels of p53-induced transcription far higher than those obtained by co-operation between P- and D-elements. These studies imply that distinct D- and P-p53-binding sites in the MCK promoter may dictate the promoter response to p53.

【 授权许可】

Unknown