Individual promoter and intron p53‐binding motifs from the rat Cyclin G1 promoter region support transcriptional activation by p53 but do not show co‐operative activation

FEBS Letters

Jackson, Paul¹  Zauberman, Arie²  Oren, Moshe²  Yardley, Gina¹ 

[1] Oncology Research Centre, Prince of Wales Hospital, Randwick, NSW 2031, Australia;Department of Molecular Cell Biology, The Weizmann Institute, Rehovot 76100, Israel

关键词: p53;    Transcription;    Cyclin G1;    Promoter;    Gene activation;   

DOI  :  10.1016/S0014-5793(98)00650-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rat Cyclin G1 gene promoter contains one p53-binding motif upstream of the transcription start site, and a second motif downstream in the first intron. We have investigated the possibility that these motifs co-operate to permit high level promoter activation by p53. Although individual motifs supported p53-dependent, orientation-independent transcriptional activation, using reporter plasmids containing both motifs, we found no evidence for co-operative promoter activation either after co-transfection with human p53 expression plasmids, or after exposure of transfected cells to cisplatin and UV-radiation.

【 授权许可】

Unknown