Inositol 1,4,5‐trisphosphate receptor associated with focal contact cytoskeletal proteins

FEBS Letters

Sugiyama, Tomoyasu²  Matsuda, Yuzuru²  Mikoshiba, Katsuhiko¹ 

[1] Department of Molecular Neurobiology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan;Vessel Research Laboratory Co., Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo 194, Japan

关键词: Inositol 1;    4;    5-trisphosphate receptor;    Cytoskeletal protein;    Talin;    Focal contact;    Immunocytochemistry;    Smooth muscle cell;    IP3;    inositol 1;    4;    5-trisphosphate;    IP3R;    IP3 receptor;    ER;    endoplasmic reticulum;    PLC;    phospholipase C;    PIP2;    phosphatidylinositol bisphosphate;    FITC;    fluorescein isothiocyanate;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(99)01732-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The linkage between inositol 1,4,5-trisphosphate (IP₃) receptors (IP₃Rs) and cytoskeletal proteins is considered to be important in cell function. In the present study, the association of IP₃R subtypes with cytoskeletal proteins was examined using monoclonal antibodies specific to each IP₃R subtype. We found that IP₃R type 2 colocalized with talin, a focal contact cytoskeletal protein. IP₃R type 2 exhibited a patchy distribution in the peripheral cytoplasm differently from type 1 and type 3 IP₃R. Furthermore, IP₃R subtypes co-immunoprecipitated with talin, vinculin and α-actin, but not α-actinin or paxillin.

【 授权许可】

Unknown