Identification and characterization of the RNA helicase activity of Japanese encephalitis virus NS3 protein

FEBS Letters

Morikawa, Shigeru¹  Utama, Andi²  Miyamura, Tatsuo¹  Igarashi, Akira³  Morita, Kouichi³  Hasebe, Futoshi³  Hatsu, Masahiro²  Shimizu, Hiroyuki¹  Takamizawa, Kazuhiro² 

[1] Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo 208-0011, Japan;Department of Bioprocessing, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan

关键词: Japanese encephalitis virus;    RNA helicase;    NS3 protein;    ATPase;   

DOI  :  10.1016/S0014-5793(99)01705-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The NS3 protein of Japanese encephalitis virus (JEV) contains motifs typical of RNA helicase/NTPase but no RNA helicase activity has been reported for this protein. To identify and characterize the RNA helicase activity of JEV NS3, a truncated form of the protein with a His-tag was expressed in Escherichia coli and purified. The purified JEV NS3 protein showed an RNA helicase activity, which was dependent on divalent cations and ATP. An Asp-285-to-Ala substitution in motif II of the JEV NS3 protein abolished the ATPase and RNA helicase activities. These results indicate that the C-terminal 457 residues are sufficient to exhibit the RNA helicase activity of JEV NS3.

【 授权许可】

Unknown