FEBS Letters | |
Identification and characterization of the RNA helicase activity of Japanese encephalitis virus NS3 protein | |
Morikawa, Shigeru1  Utama, Andi2  Miyamura, Tatsuo1  Igarashi, Akira3  Morita, Kouichi3  Hasebe, Futoshi3  Hatsu, Masahiro2  Shimizu, Hiroyuki1  Takamizawa, Kazuhiro2  | |
[1] Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo 208-0011, Japan;Department of Bioprocessing, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan | |
关键词: Japanese encephalitis virus; RNA helicase; NS3 protein; ATPase; | |
DOI : 10.1016/S0014-5793(99)01705-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The NS3 protein of Japanese encephalitis virus (JEV) contains motifs typical of RNA helicase/NTPase but no RNA helicase activity has been reported for this protein. To identify and characterize the RNA helicase activity of JEV NS3, a truncated form of the protein with a His-tag was expressed in Escherichia coli and purified. The purified JEV NS3 protein showed an RNA helicase activity, which was dependent on divalent cations and ATP. An Asp-285-to-Ala substitution in motif II of the JEV NS3 protein abolished the ATPase and RNA helicase activities. These results indicate that the C-terminal 457 residues are sufficient to exhibit the RNA helicase activity of JEV NS3.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020308836ZK.pdf | 206KB | download |