The role of phosphatidylinositide‐3‐kinase in basal mitogen‐activated protein kinase activity and cell survival

FEBS Letters

Peppelenbosch, Maikel P¹  van Deventer, Sander J.H¹  Evertzen, Maaike W.A¹  Versteeg, Henri H¹ 

[1] Laboratory for Experimental Internal Medicine, Academic Medical Centre, G2-130, Meibergdreef 9, NL-1105 AZ Amsterdam, The Netherlands

关键词: PI 3-kinase;    Constitutive MAPK activation;    Wortmannin;    LY294002;    Cell survival;   

DOI  :  10.1016/S0014-5793(99)01701-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphatidylinositide-3-OH-kinase (PI 3-kinase) is an upstream activator of p42/p44 mitogen-activated protein kinase (MAPK), but the role of PI 3-kinase-dependent MAPK remains obscure. Here we demonstrate that in a variety of different cell types, PI 3-kinase inhibition results in an inhibition of MAPK in unstimulated cells but does not interfere with growth factor-, or TPA-induced MAPK activity. Furthermore, inhibition of either PI 3-kinase or MEK/MAPK results in cell death in serum-starved cells. We concluded that basal, but not induced MAPK activity is mediated by PI 3-kinase and that this PI 3-kinase-mediated MEK/MAPK activity is essential for cell survival in quiescent cells.

【 授权许可】

Unknown