Phosphatidylinositol 3‐kinase regulation of fluid phase endocytosis

FEBS Letters

Thorpe, Catherine¹  Jones, Arwyn T.¹  Clague, Michael J.¹ 

[1] Physiological Laboratory, University of Liverpool, PO Box 147, Crown St., Liverpool L69 3BX, UK

关键词: PI 3-kinase;    Endocytosis;    Wortmannin;    Receptor down-regulation;    Dynamin;   

DOI  :  10.1016/0014-5793(95)00576-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endocytosis of the fluid phase marker, horse radish peroxidase, into baby hamster kidney cells is inhibited by treatment of cells with the fungal metabolite wortmannin. The IC₅₀ of approximately 5 nM is consistent with the well-described action of wortmannin upon phosphatidylinositol (PI) 3-kinase. Analysis of the kinetics of uptake indicates a > 50% decrease in the initial rate of marker internalisation, a concomitant decrease in the volume of the early endosome and an increased efficiency of recycling of that marker which is internalised. As PI 3-kinase binds to activated growth factor receptors our data suggest that receptor activation can be coupled to receptor internalisation (down regulation) by localising PI 3-kinase stimulation of endocytosis.

【 授权许可】

Unknown