Hypoosmolarity influences the activity of transcription factor NF‐κB in rat H4IIE hepatoma cells

FEBS Letters

Häussinger, Dieter¹  Cariers, Alexandra¹  Schliess, Freimut¹  Michalke, Manuela¹ 

[1] Department of Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University, Moorenstr. 5, D-40225 Düsseldorf, Germany

关键词: Anisoosmolarity;    NF-κB;    Signal transduction;    Apoptosis;    Hepatoma cell;    EMSA;    electrophoretic mobility shift assay;    PARP;    poly(ADP-ribose)polymerase;    PKC;    protein kinase C;    PMA;    phorbol 12-myristate 13-acetate;    TGF-β;    transforming growth factor β;   

DOI  :  10.1016/S0014-5793(99)01719-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of anisoosmolarity on NF-κB binding activity was studied in H4IIE rat hepatoma cells. Hypoosmolarity induced a sustained NF-κB binding activity whereas the hyperosmotic NF-κB response was only minor. Hypoosmotic NF-κB activation was accompanied by degradation of the inhibitory IκB-α. Protein kinase C, PI₃-kinase, reactive oxygen intermediates and the proteasome apparently participate in mediating the hypoosmotic effect on NF-κB. Hypoosmolarity plus PMA induced, amplified and prolonged IκB-α degradation and NF-κB binding activity. Transforming growth factor β-induced apoptosis was diminished by hypoosmolarity. However, this anti-apoptotic effect was probably not related to NF-κB activation.

【 授权许可】

Unknown