FEBS Letters | |
Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1. Anti‐allergen IgG can enhance the anaphylactic reaction | |
Steinberger, Peter3  Eibensteiner, Petra B.3  Visco, Vincenzo4  Denépoux, Stéphane4  Lebecque, Serge4  Banchereau, Jacques1  Kraft, Dietrich3  Valenta, Rudolf3  Vrtala, Susanne3  Weyer, Anne2  | |
[1] Baylor Institute for Immunology Research, Dallas, TX, USA;Unit of Immuno-Allergy Pasteur Institute, Paris, France;Institute of General and Experimental Pathology, AKH, University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria;Laboratory for Immunological Research, Schering-Plough, Dardilly, France | |
关键词: Allergy; Antibody; Antigen-peptide-epitope; Molecular biology; Immunotherapy; | |
DOI : 10.1016/S0014-5793(99)01703-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report the molecular characterization of five human monoclonal antibodies, BAB1–5 (BAB1: IgG1; BAB4: IgG2; BAB2, 3, 5: IgG4), with specificity for the major birch pollen allergen, Bet v 1. BAB1–5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1 inhibited the binding of patients IgE to Bet v 1, BAB2 increased IgE recognition of Bet v 1, and, even as Escherichia coli-expressed Fab, augmented Bet v 1-induced immediate type skin reactions. The demonstration that IgG antibodies can enhance allergen-induced allergic reactions is likely to explain the unpredictability of specific immunotherapy.
【 授权许可】
Unknown
【 预 览 】
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