Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1. Anti‐allergen IgG can enhance the anaphylactic reaction

FEBS Letters

Steinberger, Peter³  Eibensteiner, Petra B.³  Visco, Vincenzo⁴  Denépoux, Stéphane⁴  Lebecque, Serge⁴  Banchereau, Jacques¹  Kraft, Dietrich³  Valenta, Rudolf³  Vrtala, Susanne³  Weyer, Anne² 

[1] Baylor Institute for Immunology Research, Dallas, TX, USA;Unit of Immuno-Allergy Pasteur Institute, Paris, France;Institute of General and Experimental Pathology, AKH, University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria;Laboratory for Immunological Research, Schering-Plough, Dardilly, France

关键词: Allergy;    Antibody;    Antigen-peptide-epitope;    Molecular biology;    Immunotherapy;   

DOI  :  10.1016/S0014-5793(99)01703-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the molecular characterization of five human monoclonal antibodies, BAB1–5 (BAB1: IgG₁; BAB4: IgG₂; BAB2, 3, 5: IgG₄), with specificity for the major birch pollen allergen, Bet v 1. BAB1–5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1 inhibited the binding of patients IgE to Bet v 1, BAB2 increased IgE recognition of Bet v 1, and, even as Escherichia coli-expressed Fab, augmented Bet v 1-induced immediate type skin reactions. The demonstration that IgG antibodies can enhance allergen-induced allergic reactions is likely to explain the unpredictability of specific immunotherapy.

【 授权许可】

Unknown