Molecular cloning and characterization of the Arabidopsis thaliana α‐subunit of elongation factor 1B

FEBS Letters

Héricourt, François¹  Jupin, Isabelle¹ 

[1]Laboratoire de Virologie Moléculaire, Institut Jacques Monod, UMR 7592, CNRS-Universités Paris 6 & Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France

关键词: Elongation factor;    α-Subunit of translation elongation factor 1B;    cDNA;    Functional complementation;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(99)01694-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a PCR-based approach, we have isolated two Arabidopsis thaliana cDNA clones (α1 and α2) encoding the α-subunit of translation elongation factor 1B (eEF1Bα). They encode open reading frames of 228 and 224 amino acids respectively, with extensive homology to eEF1Bα subunits from different organisms, particularly in the C-terminal half of the protein. They both lack a conserved phosphorylation site that has been implicated in regulating nucleotide exchange activity. Using a plasmid shuffling experiment, we demonstrated that both α1 and α2 clones are able to complement a mutant yeast strain deficient for the eEF1Bα subunit. This provides evidence that Arabidopsis encodes at least two functional isoforms of this subunit, termed eEF1Bα1 and eEF1Bα2. A third cDNA clone was isolated that appeared to result from an alternative splicing event of the eEF1Bα1 gene.

【 授权许可】

Unknown