Concurrent knock‐out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae

FEBS Letters

Boles, Eckhard¹  Krampe, Stefanie¹  Wieczorke, Roman¹  Freidel, Kerstin¹  Hollenberg, Cornelis P¹  Weierstall, Thomas¹ 

[1] Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätsstr. 1, Geb. 26.12.01, D-40225 Düsseldorf, Germany

关键词: Functional analysis;    Gene family;    Sugar transport;    Multiple deletion;    Yeast;    Gene redundancy;   

DOI  :  10.1016/S0014-5793(99)01698-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1–17, Gal2). Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Ydl247, Yjr160) are able to transport hexoses. In a yeast strain deleted for HXT1–17, GAL2, AGT1, YDL247w and YJR160c, glucose consumption and transport activity were completely abolished. However, as additional deletion of the glucose sensor gene SNF3 partially restored growth on hexoses, our data indicate the existence of even more proteins able to transport hexoses in yeast.

【 授权许可】

Unknown