Connexin43 mRNA contains a functional internal ribosome entry site

FEBS Letters

Schiavi, Adam¹  Hudder, Alice¹  Werner, Rudolf¹ 

[1]Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, P.O. Box 016129, Miami, FL 33101, USA

关键词: Connexin43;    mRNA;    Translation control;    Internal ribosome entry site;   

DOI  :  10.1016/S0014-5793(99)01699-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A reporter gene construct was used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5′-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%. A bicistronic vector assay demonstrated that the Cx43 5′-UTR contains a strong internal ribosome entry site (IRES). Deletion analysis localized the IRES element to the upstream portion of the 5′-UTR.

【 授权许可】

Unknown