The tRNA N2,N2‐dimethylguanosine‐26 methyltransferase encoded by gene trm1 increases efficiency of suppression of an ochre codon in Schizosaccharomyces pombe

FEBS Letters

Desgrès, Jean²  Costa, Annie²  Gräub, Remo¹  Schweingruber, M.Ernst¹  Niederberger, Christian¹ 

[1] Institute of General Microbiology, Baltzerstrasse 4, CH-3012 Bern, Switzerland;Centre Hospitalier Universitaire de Dijon, Dijon Cedex, France

关键词: tRNA modification;    Antisuppression;    Dimethylguanosine-26 methyltransferase;    Schizosaccharomyces pombe;   

DOI  :  10.1016/S0014-5793(99)01679-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the majority of eukaryotic tRNAs, the guanosine at position 26 is modified by a dimethyl group, but so far a function of this modification has not been detected. We isolated the Schizosaccharomyces pombe gene, trm1, encoding the tRNA N2,N2-dimethylguanosine-26 methyltransferase. Strains having the gene deleted completely lack N2,N2-dimethylguanosine. In strains carrying the weak ochre tRNA suppressor sup3-i, deletion of trm1 abolishes suppression indicating that the trm1 deletion acts as an antisuppressor mutation. The result suggests that in vivo N2,N2-dimethylguanosine-26 increases the capacity of the sup3-i serine tRNA to translate the UAA (ochre) codon.

【 授权许可】

Unknown