An antisuppressor mutant of Saccharomyces cerevisiae deficient in isopentenylated tRNA has reduced Δ2‐isopentenylpyrophosphate: tRNA‐Δ2‐isopentenyl transferase activity

FEBS Letters

Laten, Howard M.¹  Timmons, Ruth M.¹  Suid, Sopiah¹ 

[1] Loyola University of Chicago, Biology Department, 6525 North Sheridan Road, Chicago, IL 60626, USA

关键词: tRNA isopentenyltransferase;    tRNA modification;    N6-Δ2-(Isopentenyl)adenosine Saccharomyces cerevisiae;    tRNA antisuppression;   

DOI  :  10.1016/0014-5793(85)80540-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously reported the isolation and initial characterization of a mutation in Saccharomyces cerevisiae, designated mod5-1, that reduces the capacity of altered tyrosine tRNAs to suppress ochre nonsense mutations. The mutation results in the virtual elimination of the modified tRNA nucleoside, N-Δ²-(isopentenyl) adenosine, normally found adjacent to the anticodons of certain tRNA species. We demonstrate here that MOD5 codes for Δ²-isopentenylpyrophosphate: tRNA-Δ²-isopentenyl transferase, or a protein that regulates its synthesis.

【 授权许可】

Unknown