Homophilic NCAM interactions interfere with L1 stimulated neurite outgrowth

FEBS Letters

Pedersen, Nina¹  Soroka, Vladislav¹  Rønn, Lars C.B¹  Bock, Elisabeth¹  Marques, Filipe A¹  Kiselyov, Vladislav¹  Kristiansen, Lars V¹  Berezin, Vladimir¹ 

[1] Protein Laboratory, Institute of Molecular Pathology, University of Copenhagen, Panum Institute, Blegdamsvej 3C, Bld. 6.2, DK-2200 Copenhagen N, Denmark

关键词: Cell adhesion;    Neurite outgrowth;    L1;    NCAM;    Interaction;    Ig-module;   

DOI  :  10.1016/S0014-5793(99)01671-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cell adhesion molecules NCAM and L1 are considered to play key roles in neuronal development and plasticity. L1 has been shown to interact with NCAM, possibly through NCAM binding to oligomannosidic glycans present in L1. We investigated the effect of recombinant immunoglobulin (Ig) modules of NCAM involved in homophilic NCAM binding, on L1 induced neurite outgrowth from PC12-E2 cells and found a complete inhibition of L1 induced neurite outgrowth after addition of Ig-modules 1, 2 and 3 of NCAM, suggesting that the ligation state of NCAM is crucial for normal L1 signaling.

【 授权许可】

Unknown