Cloning and heterologous expression of NAD(P)H:quinone reductase of Arabidopsis thaliana, a functional homologue of animal DT‐diaphorase

FEBS Letters

Sparla, Francesca¹  Tedeschi, Gabriella²  Trost, Paolo¹  Pupillo, Paolo¹ 

[1] Dipartimento di Biologia evoluzionistica sperimentale, Università di Bologna, Via Irnerio 42, I-40126 Bologna, Italy;Istituto di Fisiologia veterinaria e Biochimica, Università di Milano, Via Celoria 10, I-20133 Milano, Italy

关键词: NAD(P)H:quinone reductase;    DT-diaphorase;    Flavoprotein;    Arabidopsis thaliana;    NQR;    NAD(P)H:quinone reductase;    AtNQR;    Arabidopsis thaliana NAD(P)H:quinone reductase;    DQ;    duroquinone;    NtNQR;    Nicotiana tabacum NAD(P)H:quinone reductase;    IPTG;    isopropyl-1-thiol-β-D-galacto-pyranoside;   

DOI  :  10.1016/S0014-5793(99)01625-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In higher plants, NAD(P)H:quinone reductase (NQR) is the only flavoreductase known to reduce quinone substrates directly to hydroquinones by a two-electron reaction mechanism. This enzymatic activity is believed to protect aerobic organisms from the oxidative action of semiquinones. For this reason plant NQR has recently been suggested to be related to animal DT-diaphorase. A cDNA clone for NQR of Arabidopsis thaliana was identified, expressed in Escherichia coli, purified and characterized. Its amino acid sequence was found related to a number of putative proteins, mostly from prokaryotes, with still undetermined function. Conversely, in spite of the functional homology, sequence similarity between plant NQR and animal DT-diaphorase was limited and essentially confined to the flavin binding site.

【 授权许可】

Unknown