Human rhinovirus HRV14 uncoats from early endosomes in the presence of bafilomycin

FEBS Letters

Blaas, Dieter¹  Prchla, Elisabeth²  Bayer, Nora²  Fuchs, Renate²  Schwab, Martin² 

[1] Institute of Biochemistry, Vienna Biocenter (VBC), University of Vienna, Dr. Bohr Gasse 9/3, A-1030 Vienna, Austria;Department of General and Experimental Pathology, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria

关键词: Human rhinovirus;    Viral uncoating;    Bafilomycin;    Endocytosis;    HRV;    human rhinovirus;    MOI;    multiplicity of infection;    TCID50;    50% tissue culture infectious dose;    p.i.;    post infection;   

DOI  :  10.1016/S0014-5793(99)01610-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Determination of infectious progeny virus and in vivo labelling with [³⁵S]methionine followed by immunoprecipitation demonstrates that the major receptor group human rhinovirus HRV14 is able to infect HeLa cells in the presence of the V-ATPase inhibitor bafilomycin A1. However, host cell shut off is delayed and viral yield is decreased in the presence of the drug. Uncoating can thus take place under conditions that prevent endosomal acidification indicating that it is catalysed by the viral receptor alone. Since transport is arrested in early endosomes upon inhibition of vesicle acidification, the data also suggest that productive uncoating takes place from early endocytic compartments.

【 授权许可】

Unknown