SMAP‐29: a potent antibacterial and antifungal peptide from sheep leukocytes

FEBS Letters

Benincasa, Monica²  Zanetti, Margherita¹  Risso, Angela¹  Gennaro, Renato²  Skerlavaj, Barbara¹ 

[1] Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, 33100 Udine, Italy;Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, Via Giorgieri, 1, 34127 Trieste, Italy

关键词: Antimicrobial peptide;    Cathelicidin;    Amphipathic helix;    Lytic peptide;    MRSA;    methicillin-resistant Staphylococcus aureus;    VREF;    vancomycin-resistant Enterococcus faecalis;    CF;    cystic fibrosis;    TFE;    trifluoroethanol;    MHB;    Mueller-Hinton broth;    MIC;    minimum inhibitory concentration;    OM;    outer membrane;    IM;    inner membrane;   

DOI  :  10.1016/S0014-5793(99)01600-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

SMAP-29 is a cathelicidin-derived peptide deduced from sheep myeloid mRNA. The C-terminally amidated form of this peptide was chemically synthesized and shown to exert a potent antimicrobial activity. Antibiotic-resistant clinical isolates highly susceptible to this peptide include MRSA and VREF isolates, that are a major worldwide problem, and mucoid Pseudomonas aeruginosa associated with chronic respiratory inflammation in CF patients. In addition, SMAP-29 is also active against fungi, including Cryptococcus neoformans isolated from immunocompromised patients. SMAP-29 causes significant morphological alterations of the bacterial surfaces, as shown by scanning electron microscopy, and is also hemolytic against human, but not sheep erythrocytes. Its potent antimicrobial activity suggests that this peptide is an excellent candidate as a lead compound for the development of novel antiinfective agents.

【 授权许可】

Unknown