A role for ADP ribosylation factor in the control of cargo uptake during COPI‐coated vesicle biogenesis

FEBS Letters

Nickel, Walter¹  Malsam, Jörg²  Gommel, Daniel²  Wieland, Felix T.² 

[1] Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA;Biochemie Zentrum Heidelberg, Ruprecht Karls-Universität Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany

关键词: COPI-coated vesicle;    Cargo;    Golgi;    Coatomer;    ADP ribosylation factor;    Vesicular transport;   

DOI  :  10.1016/S0014-5793(99)01543-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ARF-mediated hydrolysis of GTP has been demonstrated to regulate coat disassembly of Golgi-derived COPI transport vesicles (Tanigawa, G., Orci, L., Amherdt, M., Ravazzola, M., Helms, J.B. and Rothman, J.E. (1993) J. Cell Biol. 123, 1365–1371). In addition, a requirement for GTP hydrolysis at an early stage of COPI vesicle biogenesis has been established since cargo uptake is impaired in the presence of GTPγS (Nickel, W., Malsam, J., Gorgas, K., Ravazzola, M., Jenne, N., Helms, J.B. and Wieland, F.T. (1998) J. Cell Sci. 111, 3081–3090), a non-hydrolyzable analogue of GTP. We now demonstrate that the GTPase involved in the regulation of cargo uptake is ARF, revealing a multi-functional role of this GTPase in COPI-mediated vesicular transport. The molecular mechanism of cargo uptake as well as the functional implications of these findings on the overall process of COPI vesicle biogenesis are discussed.

【 授权许可】

Unknown