Yeast coatomer contains a subunit homologous to mammalian β'‐COP

FEBS Letters

Lottspeich, Friedrich²  Draken, Esther¹  Harter, Cordula¹  Wieland, Felix T.¹ 

[1] Institute of Biochemistry I, University of Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany;Genzentrum, Max-Planck-Institute of Biochemistry, Am Klopferspitz, D-82152 Martinsried, Germany

关键词: Secretory pathway;    Transport vesicle;    Coatomer;    Yeast homologue;   

DOI  :  10.1016/0014-5793(93)80487-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The homologue of the mammalian coatomer complex was isolated from yeast cytosol and separated on a modified urea-containing SDS-polyacrylamide gel system. An additional band in the 100 kDa molecular weight range appeared when compared to the protein pattern obtained in conventional Laemmli gels, exactly as observed for mammalian coatomer. Cross-reactivity with an anti-peptide antibody raised against the C-terminus of β-COP from bovine, and N-terminal sequence analysis, revealed that this protein from yeast is related to β-COf from mammals.

【 授权许可】

Unknown