Gender differences in expression of organic cation transporter OCT2 in rat kidney

FEBS Letters

Inui, Ken-ichi¹  Nakamura, Nobuhiko¹  Urakami, Yumiko¹  Saito, Hideyuki¹  Okuda, Masahiro¹  Takahashi, Kazushige¹  Hashimoto, Yukiya¹ 

[1] Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan

关键词: Organic cation transporter;    Tetraethylammonium;    Basolateral membrane;    Renal tubular secretion;    (Rat kidney);    OCT;    organic cation transporter;    OAT;    organic anion transporter;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    Ntcp;    sodium taurocholate transporting polypeptide;    oatp;    organic anion transporting polypeptide;   

DOI  :  10.1016/S0014-5793(99)01491-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The organic cation transporter (OCT) mediates translocation of various cationic molecules including drugs, toxins and endogenous substances. We examined gender differences in the expression of rat (r) OCT2 in the kidney. Slices and basolateral membrane vesicles of male rat kidney showed a higher transport activity for tetraethylammonium than those of female rat kidney. The expression levels of rOCT2 mRNA and protein in the kidney of males were much higher than those in females. There was no gender difference in mRNA expression of rOCT1 and rOCT3. These findings suggest that rOCT2 is responsible for the gender differences in renal basolateral membrane organic cation transport activity.

【 授权许可】

Unknown