Unitary exocytotic and endocytotic events in guard‐cell protoplasts during osmotically driven volume changes

FEBS Letters

Homann, Ulrike¹  Thiel, Gerhard¹ 

[1] Albrecht-von-Haller Institute of Plant Sciences, University of Göttingen, Untere Karspüle 2, D-37073 Göttingen, Germany

关键词: Cell swelling-shrinking;    Exocytosis;    Endocytosis;    Guard-cell;    Membrane capacitance;    Membrane tension;    GCP;    guard-cell protoplast;    C m;    membrane capacitance;    G;    conductance;   

DOI  :  10.1016/S0014-5793(99)01396-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Osmotically driven swelling and shrinking of guard-cell protoplasts (GCPs) requires adjustment of surface area which is achieved by addition and removal of plasma membrane material. To investigate the mechanism for adaptation of surface area we have used patch-clamp capacitance measurements. The recorded membrane capacitance (C _m) trace of swelling and shrinking GCPs occasionally revealed discrete upward and downward deflecting capacitance steps, respectively, with a median value of about 2 fF. The observed capacitance steps resulted from the fusion and fission of single vesicles with a diameter of around 300 nm. We conclude that exo- and endocytosis of these vesicles accommodate for osmotically driven surface area changes in GCPs.

【 授权许可】

Unknown