Vitronectin and its fragments purified as serum inhibitors of Staphylococcus aureus γ‐hemolysin and leukocidin, and their specific binding to the Hlg2 and the LukS components of the toxins

FEBS Letters

Katsumi, Harue¹  Kamio, Yoshiyuki¹  Kaneko, Jun¹  Tomita, Toshio¹ 

[1] Department of Molecular and Cellular Biology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan

关键词: Staphylococcal γ-hemolysin;    Staphylococcal leukocidin;    Pore-forming toxin;    Serum inhibitor;    Vitronectin;    Hlg;    γ-hemolysin from Staphylococcus aureus;    Hlg1 and Hlg2;    the Hlg1 and Hlg2 components of staphylococcal γ-hemolysin;    Luk;    leukocidin from Staphylococcus aureus;    LukS and LukF;    the LukS and LukF components of staphylococcal leukocidin;    PBS;    phosphate-buffered saline;    PMSF;    phenylmethylsulfonyl fluoride;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(99)01376-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Staphylococcal γ-hemolysin and leukocidin are bi-component cytolysins, consisting of LukF (or Hlg1)/Hlg2 and LukF/LukS, respectively. Here, we purified serum inhibitors of γ-hemolysin and leukocidin from human plasma. Protein sequencing showed that the purified inhibitors of 62, 57, 50 and 38 kDa were the vitronectin fragments with truncation(s) of the C-terminal or both N- and C-terminal regions. The purified vitronectin fragments specifically bound to the Hlg2 component of γ-hemolysin and the LukS component of leukocidin to form high-molecular-weight complexes with them, leading to inhibition of the toxin-induced lysis of human erythrocytes and human polymorphonuclear leukocytes, respectively. Intact vitronectin also showed inhibitory activity to the toxins. The ability of γ-hemolysin and leukocidin to bind vitronectin and its fragments is a novel function of the pore-forming cytolysins.

【 授权许可】

Unknown