FEBS Letters | |
Vitronectin and its fragments purified as serum inhibitors of Staphylococcus aureus γ‐hemolysin and leukocidin, and their specific binding to the Hlg2 and the LukS components of the toxins | |
Katsumi, Harue1  Kamio, Yoshiyuki1  Kaneko, Jun1  Tomita, Toshio1  | |
[1] Department of Molecular and Cellular Biology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan | |
关键词: Staphylococcal γ-hemolysin; Staphylococcal leukocidin; Pore-forming toxin; Serum inhibitor; Vitronectin; Hlg; γ-hemolysin from Staphylococcus aureus; Hlg1 and Hlg2; the Hlg1 and Hlg2 components of staphylococcal γ-hemolysin; Luk; leukocidin from Staphylococcus aureus; LukS and LukF; the LukS and LukF components of staphylococcal leukocidin; PBS; phosphate-buffered saline; PMSF; phenylmethylsulfonyl fluoride; PAGE; polyacrylamide gel electrophoresis; | |
DOI : 10.1016/S0014-5793(99)01376-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Staphylococcal γ-hemolysin and leukocidin are bi-component cytolysins, consisting of LukF (or Hlg1)/Hlg2 and LukF/LukS, respectively. Here, we purified serum inhibitors of γ-hemolysin and leukocidin from human plasma. Protein sequencing showed that the purified inhibitors of 62, 57, 50 and 38 kDa were the vitronectin fragments with truncation(s) of the C-terminal or both N- and C-terminal regions. The purified vitronectin fragments specifically bound to the Hlg2 component of γ-hemolysin and the LukS component of leukocidin to form high-molecular-weight complexes with them, leading to inhibition of the toxin-induced lysis of human erythrocytes and human polymorphonuclear leukocytes, respectively. Intact vitronectin also showed inhibitory activity to the toxins. The ability of γ-hemolysin and leukocidin to bind vitronectin and its fragments is a novel function of the pore-forming cytolysins.
【 授权许可】
Unknown
【 预 览 】
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