FEBS Letters | |
The C‐terminal region of the S component of Staphylococcal leukocidin is essential for the biological activity of the toxin | |
Nariya, Hirofumi1  Kamio, Yoshiyuki1  Izaki, Kazuo1  | |
[1] Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Amamiya-machi, Aoba-ku, Sendai 981, Japan | |
关键词: Staphylococcal leukocidin; S component; Monosialoganglioside GM1; S; S component of leukocidin; F; F component of leukocidin; GM1; monosialoganglioside GM1; SDS-PAGE; sodium dodecylsulfate polyacrylamide gel electrophoresis; HPLC; high-performance liquid chromatography; | |
DOI : 10.1016/0014-5793(93)80225-J | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The Staphylococcal toxin leukocidin consists of two protein components, F and S. From a culture medium of Staphylococcus aureus RIMD 310925, we isolated a truncated form of S (LS2), of which the C-terminal 17-residue segment is missing. Unlike intact S, LS2 showed neither leukocytolytic activity in the presence of F nor affinity for monosialoganglioside GM1 (GM1). When excited at 280 nm, both S and LS2 exhibited intrinsic tryptophan fluorescence with an emission maximum at 318 nm. Upon binding to GM1, the emission maximum of S underwent a blue shift to 310 nm, whereas no change in fluorescence took place on mixing GM1 with LS2. We conclude that the C-terminal region of S is essential for its biological activity as well as for its binding to GM1 and that this binding is accompanied by a conformational change of the S protein.
【 授权许可】
Unknown
【 预 览 】
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