Determination of cDNA, gene structure and chromosomal localization of the novel human 17β‐hydroxysteroid dehydrogenase type 7

FEBS Letters

Imai, Kenji²  Adamski, Jerzy²  Breitling, Rainer²  Krazeisen, Antje²  Möller, Gabriele²  Fritz, Stephanie¹ 

[1] Technical University Munich, Institute of Anatomy, Biedersteinerstr. 29, 80802 Munich, Germany;GSF-National Research Center for Environment and Health, Institute of Mammalian Genetics, Genome Analysis Center, P.O. Box 1129, 85758 Neuherberg, Germany

关键词: 17β-Hydroxysteroid dehydrogenase;    Prolactin receptor binding;    Glioblastoma;    Alternative splicing;    Chromosome 10p11.2;    17β-HSD;    17β-hydroxysteroid dehydrogenase;    HSD17B7;    gene of human 17β-HSD 7;    SCAD;    short-chain alcohol dehydrogenase;   

DOI  :  10.1016/S0014-5793(99)01366-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified human 17β-hydroxysteroid dehydrogenase type 7 (17β-HSD 7). The novel human cDNA encodes a 37 kDa protein that shows 78 and 74% amino acid identity with rat and mouse 17β-HSD 7, respectively. These enzymes are responsible for estradiol production in the corpus luteum during pregnancy, but are also present in placenta and several steroid target tissues (breast, testis and prostate) as revealed by RT-PCR. The human 17β-HSD 7 gene (HSD17B7) consists of nine exons and eight introns, spanning 21.8 kb and maps to chromosome 10p11.2 close to susceptibility loci for tumor progression, obesity and diabetes. The HSD17B7 promoter (1.2 kb) reveals binding sites for brain-specific and lymphoid transcription factors corresponding to additional expression domains in hematopoietic tissues and the developing brain as identified by in silico Northern blot.

【 授权许可】

Unknown